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KMID : 0368420080510010074
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Journal of Plant Biology
2008 Volume.51 No. 1 p.74 ~ p.77
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Detection of gene flow from GM to non-GM watermelon in a field trial
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Kim Chang-Gi
Lee Bum-Kyu
Kim Dae-In
Park Ji-Eun
Kim Hyo-Jeong
Park Kee-Woong
Yi Hoon-Bok
Jeong Soon-Chun
Yoon Won-Kee
Harn Chee-Hark
Kim Hwan-Mook
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Abstract
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Gene flow from genetically modified (GM) crops to conventional non-GM crops is a serious concern for protection of conventional and organic farming. Gene flow from GM watermelon developed for rootstock use, containing cucumber green mottle mosaic virus (CGMMV)-coat protein (CP) gene, to a non-GM isogenic control variety “Clhalteok” and grafted watermelon “Keumcheon” was investigated in a small scale field trial as a pilot study. Hybrids between GM and non-GM watermelons were screened from 1304 “Chalteok” seeds and 856 “Keumcheon” seeds using the duplex PCR method targeting theCGMMV- CP gene as a marker. Hybrids were found in all pollen recipient plots. The gene flow frequencies were greater for “Chaiteok” than for “KeumcheonD; with 75% outcrossing in the “Chaiteok” plot at the closest distance (0.8 m) to the GM plot. A much larger scale field trial is necessary to identify the isolation distance between GM and non-GM watermelon, as the behaviors of insect pollinators needs to be clarified in Korea.
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KEYWORD
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Citrullus lanatus, gene flow, genetically modified crop, watermelon
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